The sampling procedure bacteriological water
1. Clean the faucet. Clean the faucet of any objects attached and may interfere with using a clean cloth, wipe any dust from the faucet end. 2. Opening the faucet. Turn the faucet so that the water flows to the fullest and let the water drain for 1-2 minutes.
3. Sterilize faucet Sterilize the faucet for one minute with the fire of cotton that has been dipped in alcohol, another alternative to using other burners with gas.
4. Opening the bottle - the bottle
a. Standard technique Strap in brown protective paper is then lifted or removed in turn.
b. Techniques cover the appliance. Protective strap in brown protective paper released later in the lift while other friends opened a small package contents sterile caps.
5. Filling bottles - bott While holding the cover and a protective face facing down (to prevent the entry of dust that may contain microorganisms). Bottle immediately in place under the fountain and filled the air in a bottle feel but are so biased shaken at retrieval time before analysis.
6. Or Blocked Bottle Cover
a. Engineering Standards Bottle stoppers or closed by rotating and then covered with brown paper demanteli tempelnya and tied.
b. Closing Techniques Premises Equipment The bottle stopper or lid by turning then melindngi degan dimanteli brown paper and tied in place. (Ministry of Health, Directorate General of PPM and PLP 1995)
7. Examination Method:
1. Preparation of specimens: a. For solid or liquid specimens but concentrated, diluted with distilled water or water used sterile saline or Ringer Solotion Quarter Strength. 10 grams or 10 cc of sterile distilled water specimens or other added to 100 cc. b. While the liquid specimen can be examined directly. 2. Variety LB Used: Variety 1: 5 x 10 ml, 1 x 1 ml, 1 x 0.1 ml. For specimens that are processed or expected low numbers of bacteria.
a. Liquid or dissolved specimens planted in:
- 5 Triple strength lactose broth tubes each - each 10 ml.
- 1 tube lactoce single strength broth, 1 ml.
- 1 single strength lactose broth tubes, 0.1 ml.
Put 37 º C incubator for 48 hours.
b. Each - each tube lactose broth (LB) that show positive gases, planted into brilliant green lactose bile broth (BGLB).
c. Read and recorded BGLB that show positive gases, respectively - each planted Mac Conkey order / Endo order / eosin Methyleen Blue order / Tergitol 7 that plate, input 37 º C incubator for 24 hours.
To get the MPN index of coliform, MPN tubes used by positive BGLB tubes of gas.
d. E. coli is a suspect colony grown on SIM / MIO / MIU (to determine indole production) and Simmon's citrate (to determine the ability of the bacteria to the citrate as a carbon source) and TSI agar.
Put 37 º C incubator for 24 hours.
e. Read and recorded growth in media PSI, driver's license, and the SC to determine whether or not E. coli. Then look at the table to determine the index MPN MPN E.coli.
8. Sample Reading Results
Variety 1:
5 x 10 ml tubes, BGLB (+) gas: 3)
Tube 1 x 1 ml, BGLB (+) gas: 1) MPN Index: 12
1 x 0.1 ml tubes, BGLB (+) gas: 0)
9. Note:
a. If the readings BGLB, all tubes that show the results (+) gas, planting can be forwarded by diluting the specimen 10 x or 100 x lower than the range of LB that has been done. MPN results obtained are multiplied by 10 x or 100 x.
b. MPN index calculation can also be done with Formula Thomas:
(A + B + C) x (√ (S x N) - 1 x 100 = ........
A = number of tubes (+) gas planting the first group.
B = number of tubes (+) gas planting the second group.
C = number of tubes (+) gas planting the third group.
S = number of samples grown ml.
N = number of samples negative ml.
1. Clean the faucet. Clean the faucet of any objects attached and may interfere with using a clean cloth, wipe any dust from the faucet end. 2. Opening the faucet. Turn the faucet so that the water flows to the fullest and let the water drain for 1-2 minutes.
3. Sterilize faucet Sterilize the faucet for one minute with the fire of cotton that has been dipped in alcohol, another alternative to using other burners with gas.
4. Opening the bottle - the bottle
a. Standard technique Strap in brown protective paper is then lifted or removed in turn.
b. Techniques cover the appliance. Protective strap in brown protective paper released later in the lift while other friends opened a small package contents sterile caps.
5. Filling bottles - bott While holding the cover and a protective face facing down (to prevent the entry of dust that may contain microorganisms). Bottle immediately in place under the fountain and filled the air in a bottle feel but are so biased shaken at retrieval time before analysis.
6. Or Blocked Bottle Cover
a. Engineering Standards Bottle stoppers or closed by rotating and then covered with brown paper demanteli tempelnya and tied.
b. Closing Techniques Premises Equipment The bottle stopper or lid by turning then melindngi degan dimanteli brown paper and tied in place. (Ministry of Health, Directorate General of PPM and PLP 1995)
7. Examination Method:
1. Preparation of specimens: a. For solid or liquid specimens but concentrated, diluted with distilled water or water used sterile saline or Ringer Solotion Quarter Strength. 10 grams or 10 cc of sterile distilled water specimens or other added to 100 cc. b. While the liquid specimen can be examined directly. 2. Variety LB Used: Variety 1: 5 x 10 ml, 1 x 1 ml, 1 x 0.1 ml. For specimens that are processed or expected low numbers of bacteria.
a. Liquid or dissolved specimens planted in:
- 5 Triple strength lactose broth tubes each - each 10 ml.
- 1 tube lactoce single strength broth, 1 ml.
- 1 single strength lactose broth tubes, 0.1 ml.
Put 37 º C incubator for 48 hours.
b. Each - each tube lactose broth (LB) that show positive gases, planted into brilliant green lactose bile broth (BGLB).
c. Read and recorded BGLB that show positive gases, respectively - each planted Mac Conkey order / Endo order / eosin Methyleen Blue order / Tergitol 7 that plate, input 37 º C incubator for 24 hours.
To get the MPN index of coliform, MPN tubes used by positive BGLB tubes of gas.
d. E. coli is a suspect colony grown on SIM / MIO / MIU (to determine indole production) and Simmon's citrate (to determine the ability of the bacteria to the citrate as a carbon source) and TSI agar.
Put 37 º C incubator for 24 hours.
e. Read and recorded growth in media PSI, driver's license, and the SC to determine whether or not E. coli. Then look at the table to determine the index MPN MPN E.coli.
8. Sample Reading Results
Variety 1:
5 x 10 ml tubes, BGLB (+) gas: 3)
Tube 1 x 1 ml, BGLB (+) gas: 1) MPN Index: 12
1 x 0.1 ml tubes, BGLB (+) gas: 0)
9. Note:
a. If the readings BGLB, all tubes that show the results (+) gas, planting can be forwarded by diluting the specimen 10 x or 100 x lower than the range of LB that has been done. MPN results obtained are multiplied by 10 x or 100 x.
b. MPN index calculation can also be done with Formula Thomas:
(A + B + C) x (√ (S x N) - 1 x 100 = ........
A = number of tubes (+) gas planting the first group.
B = number of tubes (+) gas planting the second group.
C = number of tubes (+) gas planting the third group.
S = number of samples grown ml.
N = number of samples negative ml.
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