Method / way Examination hemoglobin levels (Hb)

Among the most commonly used method in the laboratory and the simplest is the Sahli method , and a more sophisticated method is cyanmethemoglobin . ( Bachyar , 2002) In the Sahli method , hemoglobin dihidrolisi with HCl into globin ferroheme . Ferroheme by oxygen in the air is oxidized to ferriheme will immediately react with Cl ions forming ferrihemechlorid also called hematin or hemin brown . Color formed is compared with standard colors ( only with the naked eye ) . For ease of comparison , standard colors are made constant , which changed the color of hemin is formed . Hemin color change is made in a way such that the dilution of the same color with the standard color . Because of that compares is with the naked eye , the very subjectivity of North Sumatra University influential . In addition to currency factors , other factors , such as sharpness , exposure and so can affect the readings . Nevertheless for inspection in areas that do not have sophisticated equipment or inspection in the field , Sahli method is still adequate and trained late when examination results are reliable . More sophisticated methods are methods cyanmethemoglobin . In this method, the hemoglobin is oxidized by potassium ferrosianida into methemoglobin which then reacts with cyanide ion to form cyan - methemoglobin in red . The intensity of the color is read and compared with the standard photometer . Because comparing electronic device , then the result is more objective . However , photometers are still quite expensive , so not all laboratories have it .
Inspection procedures by the method of Sahli

    reagents :
        0.1 N HCl
        distilled water

    Equipment / facilities :
        pipette hemoglobin
        tool Sahli
        pipette pastor
        mixer

    Working procedures :
        Enter 0.1 N HCl into the tube until the number 2 Sahli
        Clean the fingertip blood to be taken with a disinfectant solution ( 70 % alcohol , betadin and so on) , then prick with a lancet or other device
        Sip with hemoglobin pipette over the limit , clean the pipette tip , and then drops of blood to mark the boundary by shifting the pipette tip to the filter paper / tissue paper .
        Insert the pipette into a tube containing blood hemoglobin , until the pipette tip attached to the bottom of the tube , then blow gently . Try to keep the air bubbles do not arise . Rinse the rest of the blood is attached to the wall by sucking HCl pipette and blow it out again as much as 3-4 times
        Mix well and let stand for about 10 minutes .
        Put it in the comparator , dilute with distilled water dropwise until the color of the solution ( after mixing until homogeneous ) the same color as the glass of the comparator . When you are together , read hemoglobin levels on a scale tube .
Inspection procedures with the cyan - methemoglobin method

    Reagnesia :
        Ferrosianida potassium solution ( K3Fe ( CN ) 6 0.6 mmol / l
        Solution of potassium cyanide ( KCN ) 1.0 mmol / l University of North Sumatra

    Equipment / facilities :
        pipette blood
        tube cuvettes
        colorimeters

    Working procedures :
        Enter as many as 5 ml reagent mixture into cuvettes
        Take capillary blood as the Sahli method as 0.02 ml and put into cuvettes above , shake and let stand for 3 minutes
        Read the colorimeter at lambda 546

    calculation :
        Hb = absorbs x 36.8 gr/dl/100 ml
        Hb absorbs x = 22.8 mmol / l